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tlr3 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tlr3 antibody
    Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
    Tlr3 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr3 antibody/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    tlr3 antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "TLR7 Mediates HIV ‐1 Tat‐Induced Cellular Senescence in Human Astrocytes"

    Article Title: TLR7 Mediates HIV ‐1 Tat‐Induced Cellular Senescence in Human Astrocytes

    Journal: Aging Cell

    doi: 10.1111/acel.70086

    Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Activation Assay, Mutagenesis, Negative Control, Fluorescence



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    Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
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    Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
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    Image Search Results


    Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.

    Journal: Aging Cell

    Article Title: TLR7 Mediates HIV ‐1 Tat‐Induced Cellular Senescence in Human Astrocytes

    doi: 10.1111/acel.70086

    Figure Lengend Snippet: Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.

    Article Snippet: The following antibodies were used: HIV‐1 Tat antibody (ImmunoDX, cat#1302, dilution 1:1000 and/or Santa Cruz, cat#sc‐65,913, dilution 1:250), TLR7 antibody (ABonline, cat#ABIN3021247, dilution 1:250), TLR3 antibody (Thermo Fisher, cat#PA5‐20183, dilution 1:1000), TLR8 antibody (Invitrogen, cat# PA5‐20056, dilution 1:1000), TLR9 antibody (Thermo Fisher, cat# PA5‐20203, dilution 1:1000), p16‐INK4A (Proteintech, cat#10883‐1‐AP, dilution 1:500), and p21 CIP1 (cell signaling, cat#2947S, dilution 1:500).

    Techniques: Activation Assay, Mutagenesis, Negative Control, Fluorescence